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The present study was designed to investigate the effects of supplementation of the maturation media with glutamine and glycine onthe invitro maturation of immature oocytes. Different concentration of glutamine and glycine (0.5, 1, 2 mM) were used in the current study. The oocytes morphology, viability, cleavage, and blastocyst rates were the dependent variables in the present study (p<0.05). The highest morphology score was reported when using when1 mM glutamine and glycine compared to control (p<0.05). Addition of 0.5Mm glutamine and glycine not significantly differ from control group. Moreover, addition of 2Mm glutamine and glycine significantly decreased normal morphology % when compared to control group. The highest viability rate was reported when using 1mM of glutamine and glycine (p<0.05) which is significantly different than control and 0.5mM. Addition of 2mM significantly decreased viability when compared to control group. The highest cleavage rate was reported when using 1mM glutamine and glycine which was significantly increased when compared to control group (p<0.05). Addition of 0.5mM glutamine and glycine significantly increased cleavage rate when compared to control group (p<0.05). Addition of 2mMglutamine and glycine significantly decreased cleavage rate when compared to control group. The highest blastocyst rate was reported when using 1mM glutamine and glycine which significantly increased blastocyst formation rate when compared to control. Addition of 0.5mM glutamine and glycine significantly increased this parameter when compared to control group but addition of 2mM result didn’t differ from control (p>0.05). The highest morphology score, viability rate, cleavage rate and blastocyst formation rate were reported when using 1mM glutamine and glycine.
Eliza Andreazzi AnaCorresponding author Laboratory of Physiology, Department of Physiology, Federal University of Juiz de Fora, Juiz de Fora/MG - Brazil
Obesity is a worldwide epidemic that features a multifactorial syndrome characterized by a chronic positive energetic unbalance. Neonatal administration of monosodium L-glutamate (MSG) causes lesion on the arcuate nucleus of hypothalamus that led to development of obesity in the adult life in rodents characterized by a notorious accumulation of catecholamine in the adrenal medulla. The amino acid glycine induces catecholamine secretion of adrenal medulla. Thus, the objective of our work was to evaluate the possible effects of glycine administration in the MSG-obesity model in rats and investigate its impact on adrenal catecholamine medulla homeostasis. Male Wistar rats received MSG solution (4mg/g body weight) subcutaneously in the cervical area for 5 days after delivery, controls received saline solution. Animals were also divided in two groups, in which one received tap water added with glycine (0.1g/Kg) after weaning on 21st day until 90 days of life.Biometrical variables, visceral fat pads weight, total content and basal secretion of adrenal cathecolamine were evaluated. Glycine increased Lee index of all tested groups and had no effect on visceral adiposity. However, glycine treatment completely reestablished catecholamine total content and basal secretion of MSG-obese group. In conclusion, although glycine treatment apparently completely reestablishes catecholamine secretion homeostasis it is not sufficient to significant directly reduce visceral adiposity in MSG obesity model in rats.